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Fig. 5 | Respiratory Research

Fig. 5

From: C/EBP homologous protein promotes Sonic Hedgehog secretion from type II alveolar epithelial cells and activates Hedgehog signaling pathway of fibroblast in pulmonary fibrosis

Fig. 5

Interfering CHOP of AECII abrogates the pro-fibrotic effect of fibroblast. MRC5 was incubated with AECII CM for 72 h. A The expression of Collagen I, Fibronectin, α-SMA were examined by Western blotting. Shh (100 ng/mL) was used as the positive control. β-actin was used as the loading control. B Quantification of protein levels relative to control. C Representative immunofluorescence images on CM treated MRC5 by dual staining of Collagen I (green) and α-SMA (red). The experiment was repeated three times. Bar = 20 μm. D Quantification of percent pixel intensity of Collagen I and α-SMA. Each dot represents data from one field; n = 10 independent measures. E mRNA level of Collagen I, Fibronectin, and α-SMA relative to control. F The proliferation of CM treated MRC5 was examined by the percentage of Ki67 positive cells. Shh (100 ng/mL) was used as the positive control. G The proliferation of CM-treated MRC5 was examined by BrdU incorporation assay

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Respiratory Research

ISSN: 1465-993X

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