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Fig. 3 | Respiratory Research

Fig. 3

From: C/EBP homologous protein promotes Sonic Hedgehog secretion from type II alveolar epithelial cells and activates Hedgehog signaling pathway of fibroblast in pulmonary fibrosis

Fig. 3

Interfering CHOP during ER stress reduces Shh secretion of AECII. A Expression of CHOP and Shh were examined by Western blotting. β-actin was used as the loading control. Each group has 3 replicates. B Quantification of expression by measuring relative band gray value of blotting which normalized by β-actin. ns, non-significant. C Representative immunofluorescence images on treated MRC5. The experiment was repeated three times. Bar = 50 μm. D Quantification of percent pixel intensity of CHOP and Shh. Each dot represents data from one field; n = 10 independent measures. ns, non-significant. E mRNA level of CHOP and Shh relative to control (24 h post-treatment). F Kinetic measurements of the mRNA level of CHOP and Shh in AECII. (G-H) ELISA for Shh and TGFβ1 in the supernatant of AECII. Differences in the mean between TM and shCHOP/TM treatment groups were compared

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Respiratory Research

ISSN: 1465-993X

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