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Fig. 3 | Respiratory Research

Fig. 3

From: Impaired AT2 to AT1 cell transition in PM2.5-induced mouse model of chronic obstructive pulmonary disease

Fig. 3

PM2.5 is likely to influence the repair of alveolar damage by affecting the transdifferentiation of AT2 to AT1. A The culture process of mouse alveolar organoids. Scale bar = 500 μm or 100 μm as indicated. B Immunofluorescence staining of alveolar organoids in CONTROL group and PM2.5-exposed alveolar organoids for HOPX, SPC and Ki67 in the alveolar differentiation phase day 4. Scale bar = 50 μm. C Quantitative of AT1/AT2 ratio per 20 × field. D Quantitative of Ki67+/total ratio per 20 × field. E, F The mRNA expression of cell cycle related genes Cdc25c and Cyclin B1 in the alveolar differentiation phase day 4. G Immunofluorescence staining of intermediate AT2-AT1 cell process marker CLDN4 in the alveolar differentiation phase day 4. Scale bar = 50 μm. **p < 0.01, ***p < 0.001, ****p < 0.0001. Comparisons between two groups (CONTROL vs PM2.5 exposure) were analyzed by unpaired t test. Data presented are means ± SD. The experiments were repeated at least three times (n ≥ 3)

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