Fig. 3

NOX4 is associated with EphA2 expression in VILI model. A Total cell counts and protein concentrations. B Identification of cells in BALF with representative visual fields. Cells were mainly macrophages (black arrow) with a few lymphocytes (blue arrow), and neutrophils (red arrow). C Histopathologic features of hematoxylin and eosin-stained mouse lung tissues and lung injury score, showing vascular engorgement, hyaline membrane formation, alveolar wall edema, and inflammatory cell infiltration (200 × magnification). D Western blot of EphA2 and NOX4, and densitometry of EphA2 and NOX4 in response to HTV and EphA2 KO. N = 3 in NVC group and n = 5 in HTV group. *p < 0.05, **p < 0.01, and ***p < 0.001, analyzed by two-way ANOVA with post-hoc testing and Bonferroni correction. BALF bronchoalveolar lavage fluid, WT wild-type, KO knockout, NVC normal ventilation control, HTV high tidal ventilation