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Fig. 1 | Respiratory Research

Fig. 1

From: NADPH oxidase 4 signaling in a ventilator-induced lung injury mouse model

Fig. 1

NOX4 KO and NOX4 inhibitor attenuates VILI in mice. A Total cell counts and protein concentrations. B Cell differentiation in BALF with a representative visual field. Cells were mainly macrophages (black arrow) with a few lymphocytes (blue arrow) and neutrophils (red arrow). C Histopathologic features of hematoxylin and eosin-stained mouse lung tissues and lung injury score showing the extent of vascular engorgement, hyaline membrane formation, alveolar wall edema, and inflammatory cell infiltration (200 × magnification). D Relative levels of NOX4 mRNA determined by real-time PCR (n = 3, each group) and NOX4 immunohistochemistry (400 × magnification). E Inflammatory cytokine levels; IL-1β, IL-6, and IL-8 levels in lung tissue lysates. N = 8 in each group. *p < 0.05, **p < 0.01, and ***p < 0.001, analyzed by two-way ANOVA with post-hoc testing and Bonferroni correction. BALF bronchoalveolar lavage fluid, NVC normal ventilation control, KO knockout, HTV high tidal ventilation

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