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Fig. 5 | Respiratory Research

Fig. 5

From: miR-489-3p promotes malignant progression of non-small cell lung cancer through the inactivation of Wnt/β-catenin signaling pathway via regulating USP48

Fig. 5

MiR-489-3p propels the malignant phenotypes of NSCLC cells via USP48. A QPCR assessed the expression of USP48 in BEAS-2B, H1299 and A549 cells. BC CCK-8 and colony formation assays were implemented to evaluate the proliferation of H1299 and A549 cells after the inhibition of USP48. D TUNEL assays were performed to assess cell apoptosis after the silencing of USP48. EF Evaluation of H1299 and A549 cell migration was assessed by transwell and wound healing assays after USP48 ablation. G Microscope was employed to observe the shapes of H1299 and A549 cells after USP48 ablation. H Western blot evaluated the protein levels of ZO-1, E-cadherin, N-cadherin and Vimentin after USP48 interference. I Western blot assessed the levels of β-catenin after the transfection of mimics NC, miR-489-3p mimics or miR-489-3p mimics + pcDNA3.1-USP48. **P < 0.01

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