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Fig. 7 | Respiratory Research

Fig. 7

From: Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin

Fig. 7

sTSLP induced receptor complex formation of IL-7R and TSLPR as an antagonist to block signal from the lTSLP. A Lysates from16-HBE was immunoprecipitated with antibodies to IL-7R, and immunocomplexes were measure by western blot assay. Dual IF staining of IL-7R (red) and TSLPR (green) were performed to analyze interaction of IL-7R and TSLPR in 16-HBE (B) and epithelium of asthma mouse airway (C). Scale bars: 10 μm. D The phosphorylation of JAK1 and JAK2 determined by Western blot. E, F GLPG0634 (GL, a selective inhibitor of JAK1), TG101348 (TG, a selective inhibitor of JAK2) and INCB018424 (IN, a selective inhibitor of JAK1 and 2) was added respectively into 16-HBE before lTSLP or sTSLP, and phosphorylation of JAK1, JAK2 or STAT5 were determined by Western blot. G Schematic model for the different effects of lTSLP and sTSLP on IL-7R/TSLPR/JAK/STAT5 pathway. *P < 0.05, n = 3 independent experiments

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Respiratory Research

ISSN: 1465-993X

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