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Fig. 4 | Respiratory Research

Fig. 4

From: Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin

Fig. 4

miR-223 mediated lTSLP-induced HIF-1α expression by targeting VHL. A Western blots showing VHL protein expression in 16-HBE after miRNAs overexpression by infecting with five different miRNAs mimics. B–E the expression of miR-20, miR-141, miR-200 and miR-223 in HDM- or lTSLP-induced 16-HBE was detected by quantitative polymerase chain reaction analysis. F Western blots showing VHL and HIF-1α expression in 16-HBE expressing miR-223 inhibitor (inh-223) and treating with HDM or lTSLP. G The Glu, LDHA and LD were measured in 16-HBE with inh-223 transfection. H ELISA was used to measure inflammatory cytokine IL-25 and IL-33 in cell culture supernatant. I Sequences present in the 3′-UTR of VHL targeted by miR-223 and its target region are capital. J The effect of miR-223 expression on luciferase reporter gene activity when linked with the targeted segment of the 3′-UTR of VHL by luciferase assay. *P < 0.05, n = 3 independent experiments

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Respiratory Research

ISSN: 1465-993X

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