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Fig. 3 | Respiratory Research

Fig. 3

From: Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin

Fig. 3

HDM/lTSLP activated HIF-1α/LDHA pathway by restrain VHL expression. Western blots analysis showing HIF-1α and LDHA levels in 16-HBE was treated with different concentration of HDM (A) and lTSLP (B). C HIF-1α expression was knockdown by siRNA and was measure by western blots. After knockdown of HIF-1α expression and pretreatment with HIF-1α inhibitor BAY-872247 (BAY), HDM- (D) or lTSLP- (E) induced LDHA expression were measured by western blots. Western blots analysis showing PHD and VHL levels in 16-HBE was treated with different concentration of HDM (F) and lTSLP (G). H 16-HBE was infected with lentivirus to overexpress VHL (OE-VHL). I After overexpression of VHL, HDM (400 U/ml)- or lTSLP (10 ng/ml)-induced HIF-1α and LDHA expression were measure by western blot assay. J A series of metabolic parameters was measured for Glu, LDHA and LD in 16-HBE with VHL overexpression. K ELISA was used to measure inflammatory cytokine IL-25 and IL-33 in cell culture supernatant. *P < 0.05, n = 3 independent experiments

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