Fig. 5

Immunofluorescence of α-SMA, SPC, AQP5, and hM in the lung section of each group after bleomycin instillation and cells transplantation. The right lobes of lungs were cut into slices with a thickness of 5–7 μm, incubated with suitable antibodies for IFC staining. A Anti-α-SMA antibody (red), anti-SPC antibody (green), and DAPI (blue) staining showed the degree of fibrosis and expression of SPC in the lung tissues of mice. B Staining with antibodies of SPC (red) and human mitochondria (hM, green) that showing survival of MSCs or BCs, and differentiation into type II alveolar epithelial cells in the lung tissues of mice. C Staining with antibodies of AQP5 (red) and hM (green) that showing survival of MSCs or BCs, and differentiation into type I alveolar epithelial cells in the lung tissues of mice. Mouse pulmonary epithelial cells only shows green fluorescence in cytoplasm, while undifferentiated human MSCs or BCs with only red fluorescence, and differentiated MSCs or BCs displays overlapping positive staining of green and red fluorescence (white arrow). Scale bars: 100 μm. n = 6 per group