Fig. 1

Culture and identification of BCs. BCs were primarily isolated and cultured from airway mucosal biopsy samples from non-fibrotic patients, with DMEM/F12 medium supplement with Y-27632, A-83-01, DMH-1, and CHIR99021. A The cultured BCs were observed under a light microscope. B BCs was stained by two cell makers, P63 (Green) and KRT5 (Red). DAPI was used for nuclear staining of BCs. Scale bars: 50 μm