Fig. 3

Production of total, anti-NTHi and anti-dsDNA immunoglobulin (Ig) G in BAL (A–C) and serum (D–F) in mice either vitamin D sufficient or deficient exposed to cigarette smoke or room air for 14 weeks (series 1). Total IgG (A, D) is expressed as µg/ml. Anti-NTHi and anti-dsDNA IgG are expressed in arbitrary units. ^$$$p < 0.001 (two-way ANOVA, sufficient vs deficient); ⁺⁺⁺p < 0.001, ⁺⁺⁺⁺p < 0.0001 (two-way ANOVA, air vs CS); *p < 0.05, **p < 0.01, ***p < 0.001 (Bonferroni post-hoc test) and φ: p < 0.05 (unpaired T-test, sufficient vs deficient). Data are expressed as median ± IQR, with n = 8–15 mice/group