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Fig. 4 | Respiratory Research

Fig. 4

From: The relation between age and airway epithelial barrier function

Fig. 4

Association between age and epithelial barrier function in vitro. A Transepithelial resistance (TER) of fully differentiated air–liquid interface (ALI) cultures of healthy subjects below 45 years of age and above 45 years of age. Primary bronchial epithelial cells (PBECs) were isolated from healthy subjects of different age and cultured at ALI for 21 days and differences between the age group were tested with the Mann–Whitney U test, * = p < 0.05. B Spearman correlation between TER of the ALI cultures and age. C Spearman correlation between TER of ALI cultures and expression of CDH1, presented as the log2 fold of the delta Ct value. D Real-time measurement of the epithelial resistance of control (blue line), CDH1+/− (red) and CDH1−/− (green) CRISPR–Cas9 induced knockout 16HBE14o-cells for 48 h. The mean of n = 3 for each group is shown, and the faint dotted lines represent the SEM. Differences between the groups were tested with 2way-ANNOVA, * = p < 0.05

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