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Fig. 1 | Respiratory Research

Fig. 1

From: Early life exposure to nicotine modifies lung gene response after elastase-induced emphysema

Fig. 1

Elastase instillation decreased mouse weight at ped3 and increased lung volume at ped21. The 5 different lobes of saline (control, left) and elastase-instilled (right) lungs were imaged with synchrotron X-ray tomographic microscopy (A). White arrows point out the areas with extensive emphysema in elastase-instilled lungs. Weight loss of mice after saline or elastase instillation was measured at ped3 (B) and lung volume was measured at ped21 (C) for all 4 groups of animals: control-saline (C-S, n = 14 animals for the weight and 7 animals for lung volume respectively), control-elastase (C-E, n = 20 and 7 animals), nicotine-saline (N-S, n = 13 and 7 animals) and nicotine-elastase (N-E, n = 20 and 8 animals). Weight loss was significantly higher in elastase-instilled animals compared to saline-instilled animals (p < 0.05) by two-way ANOVA independently of nicotine pretreatment. No significant difference was present between the 4 groups (B). By three-way ANOVA, instillation of elastase significantly affected the lung volume (p < 0.001). Two-way ANOVA analysis between individual groups in both control and nicotine background showed a significant increase of lung volume with p < 0.01 (C)

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