Fig. 4

Effect of blocking FAO on intermittent CS-induced activation of AT2 stemness. A Study design and experimental protocol. The carnitine palmitoyltransferase (Cpt1), inhibitor etomoxir (25 mg/kg) or vehicle (normal saline) was intraperitoneally injected into intermittent CS-exposed Sftpc/GFP mice every other day for 5 weeks. B–D Representative day 14 images are presented for intermittent CS-exposed mice treated with normal saline i.p. (B) and etomoxir i.p. (C). Colony-forming efficiency (CFE) was inhibited in the etomoxir group (D). Quantification of CFE was conducted using triplicate wells. Data are presented as means ± SD. The reproducibility was confirmed using two repeated experiments. *P < 0.05. Scale bars: 500 μm