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Fig. 3 | Respiratory Research

Fig. 3

From: Short-term intermittent cigarette smoke exposure enhances alveolar type 2 cell stemness via fatty acid oxidation

Fig. 3

Differentially regulated genes in AT2 cells exposed to continuous and intermittent CS. For each group, AT2 cells were pooled together from three mice, and used for microarray analysis and real-time PCR. A Top 25 upregulated and downregulated genes identified in the microarray analysis in continuous CS-exposed and intermittent CS-exposed groups. The top three genes in the intermittent CS-exposed group (red boxes) are related to fatty acid metabolism. B and C Confirmation of several of the upregulated genes by real-time PCR. Data are presented as relative expression compared to levels in AT2 cells isolated from continuous CS-exposed or intermittent CS-exposed lungs. D–G Representative images of cells immunofluorescence-stained for Cpt1a in cytospin slides from air-exposed (D), continuous CS-exposed (E), and intermittent CS-exposed groups (F). The ratio of Cpt1a-stained area per cell was significantly higher in the intermittent CS-exposed group than in the air-exposed and continuous CS-exposed group. The ratio in each group was calculated based on the average value of the air-exposed group. Data are presented as means ± SD. N = 35 (air), 52 (continuous CS exposure) and 89 (intermittent CS exposure). *P < 0.05. Scale bars: 50 μm

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