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Fig. 8 | Respiratory Research

Fig. 8

From: Nifuroxazide ameliorates pulmonary fibrosis by blocking myofibroblast genesis: a drug repurposing study

Fig. 8

NIF suppresses EMT and migration of A549 cells induced by TGF-β1. a Viability of A549 cells after treatment with different concentrations of NIF for 24, 48 or 72 h. Statistical significance was assessed using Student’s t test; each point represents the mean ± SD of three independent experiments per condition; *P < 0.05; **P < 0.01; ***P < 0.001 vs. vehicle control. b Representative images of migration in A549 treated with TGF-β1 or NIF for 24 h. Scale bars, 100 µm. c Quantified wound healing area ratio. d Representative fluorescence staining images of E-Cadherin (red) and nuclei (blue) in A549 cells treated with TGF-β1 or NIF for 24 h. Scale bars, 20 µm. e Representative immunoblots of α-SMA, E-Cadherin, Vimentin, p-Smad2/3, Smad2/3, and GAPDH from A549 cells treated with TGF-β1 or NIF for 24 h. f Bar graph showing the mean data from all subjects analysed in each group in e. Statistical significance was assessed using one-way ANOVA followed by Tukey’s test in c, f. All data are shown as the mean ± SD of three independent experiments per condition; **P < 0.01; ***P < 0.001 vs. DMSO; #P < 0.05, ##P < 0.01 vs. TGF-β1

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