Fig. 6

NIF reverses pulmonary fibrosis induced by BLM. a Representative images showing H&E and Masson’s trichrome staining. Scale bars, 100 μm. b Quantification of fibrosis in lung sections based on the results of H&E staining. c Quantification of the collagen content volume fraction of lung sections based on Masson staining results. d Representative images showing α-SMA and Collagen I staining of lung sections in mice. Scale bars, 50 µm. e Representative immunoblots of α-SMA, Collagen I, E-Cadherin, Vimentin, and β-actin in lung homogenates of mice as indicated. NIF (50 mg/kg). Statistical significance was assessed using one-way ANOVA followed by Tukey’s test. All data are shown as the mean ± SD; n = 3–5; **P < 0.01, ***P < 0.001 vs. Sham; ^#P < 0.05 vs. Vehicle