Fig. 2

NIF reduces inflammation and expression of fibrotic markers in BLM-induced lung fibrosis. a Experimental outline for NIF preventing fibrosis after BLM-induced lung injury in mice: n = 10 (Sham); n = 14 (Vehicle); n = 10 (NIF 25 mg/kg); n = 14 (NIF 50 mg/kg). b Lung weight coefficient of mice depicted in a. c Content of hydroxyproline in lung homogenates from the groups of mice depicted in a. d–g Analysis of TNF-α, IL-4, IL-6, and TGF-β1 levels in BALF from control and BLM-treated mice in the presence of vehicle and NIF. Statistical significance was evaluated using one-way ANOVA followed by Tukey’s test. All data are shown as the mean ± SD; n = 3–5 per group; ***P < 0.001 vs. Sham; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. Vehicle