Fig. 1

Tollip deficiency promotes STAT3 activation and inflammatory response to IL-13. A Western blot image showing Tollip knockdown (KD) using the shRNA approach in tracheobronchial epithelial cells. Data was normalized to the scrambled control. B Increased STAT3 activation in Tollip KD cells vs. scrambled control after 24 h of IL-13 treatment. Data was normalized to the scrambled control cells with IL-13 treatment. C Significantly higher eotaxin-3 levels in Tollip KD cells vs. scrambled control cells after 48 h of IL-13 treatment. P-values represent a paired student’s t-test. Data are from four healthy human donors. Each dot represents one donor. Data from one donor is the mean of three technical replicates