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Fig. 2 | Respiratory Research

Fig. 2

From: Tetracycline ameliorates silica-induced pulmonary inflammation and fibrosis via inhibition of caspase-1

Fig. 2

Tetracycline inhibits activation of caspase-1. Murine BMDM were stimulated as described in Fig. 1. Immunoblots of lysates (LY; caspase-1 (p45), ASC, pro-IL-1ß, NLRP3 and ß-Actin) and supernatants (SN; caspase-1 (p20) and mature IL-1ß) of wt BMDM (A). Representative blots from ≥ 3 independent experiments. Quantification of signal intensity of caspas-1 (p20) B and mature IL-1ß (C). NLRP3 (B), ASC (C), Casp-1 D and IL-1ß E mRNA levels were determined by qPCR using 18 s RNA as an endogenous control 3 independent experiments. Median with interquartile range of ≥ 3 independent experiments. Ranksum-testing adjusting for multiple comparison after Kruskal–Wallis test

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