Fig. 2

Notch4 signaling contributes to hypoxia-induced HPASMCs proliferation, migration and apoptosis resistance. A Cell viability was performed using Cell Counting Kit-8 assay in HPASMCs. (n = 5) B, C Cell proliferation was assessed by Edu assay. (n = 3) Magnification, × 100; Bar, 100 μm. D Cell migration was performed using Transwell assay. Magnification, × 100; Bar, 50 μm. (n = 4) E Cell apoptosis was assessed by Annexin-V/propidium iodide (PI) staining. Analyses of apoptosis including early apoptosis (Annexin-V positive and PI negative) and late apoptosis (Annexin-V positive and PI positive) were shown. (n = 4) F Protein levels of Notch4, MMP9, Bcl-2, Bax, PCNA, survivin were shown. (n = 4) Data were presented as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, comparison with normoxic HPASMCs treated with si-NC; ^#P < 0.05, ^###P < 0.001, ^####P < 0.0001, comparison with hypoxic HPASMCs treated with si-NC. One-way ANOVA followed by Tukey’s correction for post-hoc comparisons were performed for A–F. CCK-8 cell counting kit-8, si-NC negative control short interfering RNAs (siRNA), si-Notch4 siRNA against Notch4