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Fig. 3 | Respiratory Research

Fig. 3

From: BTB and CNC homology 1 (Bach1) induces lung cancer stem cell phenotypes by stimulating CD44 expression

Fig. 3

Role of Bach1 in the determination of lung CSC properties. A CD44, Sox2, Nanog, and Oct4 mRNA expression in lung CSCs. B CD44, Sox2, Nanog, and Oct4 protein expression in lung CSCs. C A tumor spheroid assay was carried out to evaluate cell sphere formation ability. D ChIP-qPCR was conducted using anti-Bach1 or anti-CD44 antibodies. E Analysis of CD44 promoter activity. Cells were transfected with a Bach1-FLAG plasmid for 24 h. *P < 0.05

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