Fig. 2

Role of Bach1 in lung CSC proliferation, apoptosis, and invasion. CD44 + cells were cultured at 70% confluency and transfected with LV-shBach1 for 48 h. A The EdU assay was performed 48 h post-transfection to determine cell proliferation. B A colony formation assay was performed to assess cell colony formation capacity. C Flow cytometry was utilized to detect cell apoptosis. D p53, BCL2 and BAX protein expression in lung CSCs. E A Transwell invasion assay was performed to determine cell invasion ability. *P < 0.05; **P < 0.01