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Fig. 5 | Respiratory Research

Fig. 5

From: Role of Secretoglobin+ (club cell) NFκB/RelA-TGFβ signaling in aero-allergen-induced epithelial plasticity and subepithelial myofibroblast transdifferentiation

Fig. 5

NFκB/RelA signaling directly activates the TGFB1 promoter in response to CDE. A DOX regulated RelA shRNA-expressing hSAECs were cultured in the presence of DOX and stimulated treated in the absence or presence of CDE. Abundance of TGFβ1 mRNA was determined by Q-RT-PCR. **, p < 0.01, post hoc t-test for n = 4–6 biological replicates. B Chromatin immunoprecipitation (XChIP) assay was conducted to quantify RELA binding to the proximal TGFβ1 promoter. Crosslinked chromatin was precipitated using anti-rabbit IgG or anti-RelA and fold enrichment of TGFb1 promoter determined by Q-gPCR. C Abundance of phospho-Ser 2 CTD RNA-Pol II on TGFb1 by XChIP. D Abundance of Total RNA Pol II; *p < 0.05 compared to Control, t-test. Shown is an individual experiment, reproduced in two independent studies

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Respiratory Research

ISSN: 1465-993X

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