Fig. 2

Cat dander extract induces epithelial plasticity and activation of NFκB/RELA. C57BL/6J mice were treated with daily challenges of CDE or PBS i.n. over 4 days. A Changes in mesenchymal markers. Relative fold changes of indicated transcripts by Q-RT-PCR of total lung RNA; *p < 0.05, compared to Control, n = 6–8/group, t-test. B IFM. Sections were stained with anti-SNAI1 Ab (red) and DAPI (blue) for nuclear staining. Representative images shown at × 20 with selected enhanced image of the epithelium. C Relative fold changes in SNAI1 FI for each treatment group (n = 6–8 animals/group). D Single cell flow cytometry using a 4-day CDE exposure model. Representative gating of cell selection, removal of debris, doublets and Live-CD45⁻ cells. [Left] a representation of SMA⁺ and CD326⁺ control cells vs [Right] SMA⁺ and CD326⁺ CDE-exposed cells. E % SMA⁺ and CD326⁺ cells in the 4-day CDE exposure model, n = 5. F Sections stained with anti-RELA Ab (red) and DAPI (blue) for nuclear staining. Representative images shown at × 20 with selected enhanced image of the epithelium. G Relative fold changes in RELA FI for each treatment group (n = 6–8 animals/group)