Fig. 1
Cat dander extract induces mucosal TGFβ. C57BL/6J mice were treated with daily challenges of CDE or PBS i.n. over 4 days. A Cell count of bronchoalveolar fluid (BALF) from mouse lung. Mean ± S.D. of BALF total cell counts (104/ml) at each time point for n = 6–8 animals/group (each symbol represents an independent animal). *p < 0.05; **p < 0.01; post hoc t-test for indicated contrast. B Differentials of BALF cell counts. Mean ± S.D. (104/ml) for macrophages, lymphocytes or neutrophils at each time point. *p < 0.05; **p < 0.01; ***p < 0.001 post hoc t-test for indicated contrast. No eosinophils were detected. C Time dependent expression of Tgfβ-1/2/3 mRNAs. Mean ± S.D.of fold change abundance for each mRNA mRNA by Q-RT-PCR of total mouse lung RNA. n = 6–8 animals/group. D Immunofluorescence microscopy (IFM). Sections were stained with anti-TGFβ1 antibody (Ab, red color) and counter-stained with DAPI (blue) to visualize nuclei. Representative images shown at 20X magnification with selected enhanced image of the epithelium. E Quantitation of TGFβ1. Fluorescence images were quantified by FIJI. Mean ± SD of fluorescence intensity (FI) for n = 6–8 animals/group. F Bioactive TGFβ1 abundance in BALF. Shown are mean ± S.D. of free TGFβ1 levels in ELISA (n = 5 animals/group). G Induction of Smad3. Relative fold changes in Smad3 mRNA by Q-RT-PCR; n = 6–8 animals/group