Table 4 Sensitivity and specificity of microbiology culture, metagenomic sequencing and qRT-PCR
Pathogen | Sensitivity (95%CI) | Specificity (95%CI) | ||||
|---|---|---|---|---|---|---|
Microbiology culture | Metagenomic sequencing | qRT-PCR | Microbiology culture | Metagenomic sequencing | qRT-PCR | |
A. baumannii | 73.53% (55.35–86.49%) | 88.23% (71.61–96.16%) | 94.12% (78.94–98.97%) | 100% (90.94–100%) | 100% (90.94–100%) | 97.96% (87.76–99.89%) |
P. aeruginosa | 68.75% (41.48–87.87%) | 75% (47.41–91.67%) | 87.5% (60.41–97.80%) | 100% (93.24–100%) | 100% (93.24–100%) | 98.51% (90.86–99.92%) |
K. pneumoniae | 53.33% (27.42–77.72%) | 73.33% (44.83–91.09%) | 66.67% (38.69–87.01%) | 90.36% (81.39–95.45%) | 97.06% (88.84–99.49%) | 100% (93.34–100%) |
S. aureus | 18.75% (4.97–46.31%) | 87.5% (60.41–97.8%) | 93.75% (67.71–99.67%) | 100% (93.24–100%) | 100% (93.24–100%) | 95.52% (86.63–98.84%) |
S. maltophilia | 50% (20.14–79.86%) | 100% (65.55–100%) | 90% (54.12–99.48%) | 100% (93.77–100%) | 94.52% (85.84–98.23%) | 97.26% (89.56–99.52%) |
S. pneumoniae | 0% (0–53.71%) | 100% (51.68–100%) | 100% (51.68–100%) | 100% (94.15–100%) | 100% (94.08–100%) | 100% (94.08–100%) |
E. coli | 0% (0–43.9%) | 100% (56.09–100%) | 100% (56.09–100%) | 100% (94–100%) | 100% (94–100%) | 94.74% (86.36–98.3%) |