Table 3 Sensitivity and specificity of non-PCR amplified sequencing and PCR amplified sequencing
Pathogen | Sensitivity (95% CI) | Specificity (95% CI) | ||||
|---|---|---|---|---|---|---|
Non-PCR amplified sequencing | PCR amplified sequencing | qRT-PCR | Non-PCR amplified sequencing | PCR amplified sequencing | qRT-PCR | |
A. baumannii | 87.5% (60.41–97.8%) | 93.75% (67.71–99.67%) | 100% (75.93–100%) | 100% (71.66–100%) | 84.62% (53.66–97.29%) | 100% (71.66–100%) |
P. aeruginosa | 66.67% (24.11–94%) | 50% (13.95–86.05%) | 100% (51.68–100%) | 100% (82.19–100%) | 100% (82.19–100%) | 100% (82.19–100%) |
K. pneumoniae | 71.43% (30.26–94.89%) | 85.71% (42.01–99.25%) | 57.14% (20.24–88.19%) | 100% (81.5–100%) | 81.82% (58.99–94.01%) | 100% (81.50–100%) |
S. aureus | 75% (35.58–95.55%) | 75% (35.58–95.55%) | 87.5% (46.68–99.34%) | 100% (80.76–100%) | 100% (80.76–100%) | 100% (80.76–100%) |
S. maltophilia | 100% (31–100%) | 100% (31–100%) | 100% (31–100%) | 100% (83.98–100%) | 100% (83.98–100%) | 100% (83.98–100%) |
S. pneumoniae | 100% (39.58–100%) | 100% (39.58–100%) | 100% (39.58–100%) | 100% (83.42–100%) | 92% (72.5–98.6%) | 100% (83.42–100%) |
E. coli | 100% (5.46–100%) | 100% (5.46–100%) | 100% (5.46–100%) | 100% (84.98–100%) | 82.14% (62.42–93.23%) | 96.43% (79.76–100%) |