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Fig. 1 | Respiratory Research

Fig. 1

From: Rapid identification of pathogens associated with ventilator-associated pneumonia by Nanopore sequencing

Fig. 1

Sample Collection. A total of 105 patients with suspected VAP, and 16 of them had insufficient clinical information or samples. Enough information and samples were successfully acquired from 89 patients, but in 6 of the samples, an insufficient amount of DNA was extracted for further experiments. Step 1: 12 samples with enough volume were separated into two parts respectively, and DNA was extracted with or without host DNA depletion; Step 2: 29 low-quality DNA samples were used to build the library with and without PCR amplification. Step 3: all 83 DNA samples were extracted after host genome depletion and sequenced with the non-PCR amplification method for further analysis. VAP: ventilator-associated pneumonia; ETA: endotracheal aspirate

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