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Table 4 Relationship of structural quantities and stereological principles

From: A short primer on lung stereology

Parameter (dimension)

Appearance in 2D section (dimension)

Probe (dimension)

Event

Measurement

Density estimate

Volume V (3)

Area A (2)

Point PT (0)

Point lies in volume (“hits” A)

Point count P(x)

VV(x) = P(x)/PT

Surface area S (2)

Boundary B (1)

Line LT (1)

Line intersects surface (“hits” B)

Intersection count I(x)

SV(x) = 2·I(x)/LT

Length L (1)

Point Q (0)

Plane AT (2)

Plane transects line (“hits” Q)

Transect count Q(x)

LV(x) = 2·Q(x)/AT

Number N (0)

Volume (Disector) AT·t (3)

Disector volume “hits” particle top

Top count Q¯(x)

NV(x) = Q¯(x)/AT·t

  1. Basic structural quantities that can be estimated (and their dimension), their appearance in single thin microscopic sections (and their dimension), the appropriate geometric probes to measure them (and their dimension), the events generated by the interaction of the probe with the structure, the counts (measurements) that result, and the formulae for calculation of densities in the reference volume. These densities (ratios) per unit reference volume have to be converted to total values by multiplying them with the total volume of the reference space (see Fig. 1). After [37]