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Fig. 5 | Respiratory Research

Fig. 5

From: A short primer on lung stereology

Fig. 5

Point and intersection counting for estimation of volume and surface area. In case volume and surface area should be estimated on the same fields of view, test points and test lines can be combined into test line segments. These test line segments contain two test points, one on each end. Test point counts (large black arrows) may fall on alveolar airspace, alveolar duct airspace or alveolar septum, thus differentiating the parenchyma into compartment volume fractions (compare Fig. 1). Intersections of test lines with alveolar surface (small red arrows) are counted for surface area estimation. From Schneider and Ochs [14]

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