Fig. 2
Estimation of the volume of the fixed lung (Step 3) according to the Archimedes principle. Based on buoyancy, the weight of the displaced fluid (e.g. saline) is measured. Since the fixed lung is lighter than the fluid, it tends to emerge and thus has to be submerged by a coiled wire connected to a lab stand. The lung and wire must not touch the wall of the beaker. The wire has a mark to adjust it to the fluid level before and during measurements to make sure it does not contribute to the measured volume. The volume of the lung (in mL or cm3) is shown as weight gain (in g)