Fig. 6

PKGII gene knockdown reversed the upregulation effect of FA on the protein expression of α/γ-ENaC in MTECs. A–B Representative Western blot bands and statistic data for PKGII knockdown. ***P < 0.001, compared with negative control (NC), n = 4. C Representative Western blot bands of PKGII and α/γ-ENaC protein expression in LPS-treated MTECs transfected with siPKGII. D–F Graphical representation of data obtained from Western blot assays for PKGII and α/γ-ENaC. Bands were quantified using gray analysis (PKGII/β-actin, α-ENaC/β-actin, and γ-ENaC/β-actin). **P < 0.01, ***P < 0.001, compared with Control group; ^&P < 0.05, compared with LPS group; ^#P < 0.05, ^##P < 0.01, compared with LPS + negative control (NC), ⁺P < 0.05, ⁺⁺P < 0.01, compared with FA + LPS group, ^$P < 0.05, compared with FA + LPS + negative control (NC), n = 4