Fig. 5

The FA increased ENaC expression was dependent on cGMP. MTECs were treated with Rp-cGMP (cGMP inhibitor, 10 μM) in the presence of FA (200 μM) and LPS (10 ng/ml) for 12 h. After incubation, the cells were harvested. PKGII and α/γ-ENaC protein expressions in the cell lysates were detected by Western blot assay. A Representative Western blot bands of PKGII and α/γ-ENaC protein expression in LPS-induced MTECs treated with Rp-cGMP. B–D Graphical representation of data obtained from Western blot assays for PKGII and α/γ-ENaC. Bands were quantified using gray analysis (PKGII/β-actin, α-ENaC/β-actin, and γ-ENaC/β-actin). ***P < 0.001, compared with Control group; ^&&P < 0.01, ^&&&P < 0.001, compared with LPS group; ^##P < 0.01, ^###P < 0.001, compared with FA + LPS group, n = 4. E Immunofluorescence images of γ-ENaC in mouse tracheal tissue. scale bar = 100 μm