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Fig. 3 | Respiratory Research

Fig. 3

From: Ferulic acid ameliorates lipopolysaccharide-induced tracheal injury via cGMP/PKGII signaling pathway

Fig. 3

Isc level and mRNA expression of ENaC is enhanced by FA in MTEC monolayers. A The MTECs were incubated with different doses of FA (0, 25, 50, 100, 200, 400, 800 μM) for 24 h, and cell counting kit-8 (CCK-8) assay was performed to examine cell viability. In addition, the FA concentration of 0 μM was used as 100%. *P < 0.05, **P < 0.01, ***P < 0.001, compared with 0 μM group, n = 5–7. B Isc traced after MTEC monolayers were treated with LPS for 12 h and FA for 24 h, then amiloride (100 μM) was applied. C Statistic ASI in MTEC monolayers. ASI was defined as the difference between the total current and the amiloride-resistant current and the initial ASI was set as 100%. D–F Relative level of ENaC mRNAs were calculated as α-, β- or γ-ENaC/GAPDH ratios. *P < 0.05, **P < 0.01, ***P < 0.001, compared with Control group; &P < 0.05, &&P < 0.01, compared with LPS group, n = 3

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