Fig. 2

Effects of FA on the proteins and transcriptional expression level of ENaC in the trachea of mice. A C57BL/J male mice were treated intraperitoneally with normal saline or FA (100 mg/kg) 12 h before and 12 h after intratracheal administration of LPS (5 mg/kg), subsequently the proteins were extracted and analyzed by Western blot. The typical bands of α- and γ-ENaC protein expression and β-actin were used as internal controls. B and C Graphical representation of data obtained from Western blot assays for α- and γ-ENaC subunits, which were quantified using gray analysis (α-ENaC/β-actin and γ-ENaC/β-actin). D–F mRNA samples were isolated from mouse models of tracheal injury. Relative levels of mRNA were calculated as α- or β- or γ-ENaC/GAPDH ratios. ***P < 0.001, compared with Control group, ^&P < 0.05, compared with LPS group, n = 4–5