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Fig. 9 | Respiratory Research

Fig. 9

From: CYP2J2 and EETs protect against pulmonary arterial hypertension with lung ischemia–reperfusion injury in vivo and in vitro

Fig. 9

Exogenous EETs inhibit TNF-α combined with anoxia/reoxygenation-induced apoptosis and mitochondrial damage in HPAECs. A Annexin V-positive HPAECs stained by Annexin V-FITC and PI were counted by flow cytometry. Lower right quadrant: number of Annexin V-positive cells for early apoptosis; upper right quadrant: number of Annexin V-positive cells for late apoptosis or necrosis; upper left quadrant: number of PI-positive cells for late apoptosis or necrosis; lower left quadrant: number of PI-positive cells for early apoptosis. B The mean percentage of Annexin V-positive cells in each group (n = 3; *P < 0.05 vs. Control group, #P < 0.05 vs. TNF-α + AR or TNF-α + AR + DMSO group, $P < 0.05 vs. TNF-α + AR + EETs group). C The mitochondrial transmembrane potential was measured by JC-1 and analyzed by flow cytometry. Upper right quadrant: cells with normal mitochondrial function, which have diminished red fluorescence. Lower right quadrant: cells with loss of mitochondrial transmembrane potential with bright green fluorescence. D The mean percentage of cells with loss of mitochondrial transmembrane potential in each group (n = 3; *P < 0.05 vs. Control group, #P < 0.05 vs. TNF-α + AR or TNF-α + AR + DMSO group, $P < 0.05 vs. TNF-α + AR + EETs group)

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