Fig. 12
Effect of CYP2J2 overexpression and exogenous EETs on PI3K/AKT signaling pathway. A CYP2J2 overexpression significantly promoted the activation of PI3K/AKT signaling pathway in vivo. The upper graph shows the representative Western blot plots of PI3K, p-AKT (Ser473) and t-AKT in rat lung tissues, and the lower graph shows the fold change of PI3K/GAPDH and p-AKT/t-AKT in each group to the relative density of Control group. Fold change was calculated by normalizing to control (fold change of control is 1). (n = 3; *P < 0.05 vs. Control, PAH or PAH + Sham group, #P < 0.05 vs. PAH + IR or PAH + IR + GFP group, $P < 0.05 vs. PAH + IR + 2J2 group). B Exogenous EETs significantly promoted the activation of PI3K/AKT signaling pathway in vitro. The upper graph shows the representative Western blot plots of PI3K, p-AKT (Ser473) and t-AKT in HPAECs, and the lower graph shows the fold change of PI3K/GAPDH and p-AKT/t-AKT in each group to the relative density of Control group. Fold change was calculated by normalizing to control (fold change of control is 1). (n = 3; *P < 0.05 vs. Control or TNF-α group, #P < 0.05 vs. TNF-α + AR or TNF-α + AR + DMSO group, $P < 0.05 vs. TNF-α + AR + EETs group)