Fig. 1
From: Knockdown of circFOXO3 ameliorates cigarette smoke-induced lung injury in mice
CS treatment increased the level of circFOXO3 in the lungs of model mice and in MLE12 cells. A The circFOXO3 expression levels in the lung parenchyma samples from CS-exposed and not CS-exposed mice (n = 6) were determined by qRT-PCR. B qRT-PCR analysis of the expression of circFOXO3 in MLE12 cells treated with various concentrations of CSE for 24 h. C The qRT-PCR data show high abundance of circFOXO3 in either the cytoplasm or nucleus of MLE12 cells. GAPDH mRNA served as an internal control for the cytoplasmic RNAs. D RNA FISH for circFOXO3. E qRT-PCR analysis of the circFOXO3 expression in MLE12 cells infected with the shcircFOXO3-expressing lentivirus. *P < 0.05, **P < 0.01