Fig. 1

DEL-1 deficiency developed emphysema and augmented cigarette smoke extract (CSE)-induced emphysema in mice. A RT-PCR for DEL-1 and GAPDH in lung tissues from WT and DEL-1 KO C57BL/6 mice. B WT and DEL-1 KO mice were treated with saline or CSE as described in “Methods” (WT saline; n = 8, WT CSE; n = 8, KO saline; n = 5, KO CSE; n = 8). C Lung tissue sections were stained with H&E. Representative photographs of lungs. D MLIs are presented as mean ± S.E. (WT saline vs WT CSE, P = 0.028; WT saline vs KO saline, P = 0.003; KO saline vs KO CSE, P = 0.037; WT CSE vs KO CSE, P < 0.001) DEL-1 developmental endothelial locus-1, RT-PCR  reverse transcriptase polymerase chain reaction, GAPDH  glyceraldehyde 3-phosphate dehydrogenase, WT  wild-type, KO  knockout, H&E  hematoxylin and eosin, MLI  mean linear intercept