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Fig. 4 | Respiratory Research

Fig. 4

From: Small-molecule Akt-activation in airway cells induces NO production and reduces IL-8 transcription through Nrf-2

Fig. 4

SC79 upregulates total Nrf-2 protein and downstream targets HO-1 and NQO-1. a, b A549s were treated as in Fig. 3, followed by Western for Nrf-2. Bars are means ± SEM; n ≥ 3 experiments. a *p < 0.05 (CTL vs. SC) 2 h and b *p < 0.05 (CTL vs. SC vs. SC + LY) 2 h by ANOVA and Bonferroni posttest. c A549s were grown and treated with SC79 (10 μg/ml) for 24 h before lysing for qPCR to measure Nrf-2 (NFE2L2), HO-1, and NQO-1. Bars show expression relative to GAPDH (2−ΔCT; mean ± SEM; n = 4 experiments. *p < 0.05 (NFE2L2), **p < 0.01 (HO-1) and ***p < 0.001 (NQO-1) by unpaired t-test. Raw ΔCT values (control vs SC) were 5.48 ± 0.26 vs 4.32 ± 0.40 (NFE2L2; p < 0.05 by unpaired t-test), 3.35 ± 0.28 vs 1.75 ± 0.12 (NQO-1; p < 0.001 by unpaired t-test) 6.09 ± 0.15 vs 4.87 ± 0.23 (HO-1; p < 0.01 by unpaired t-test)

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