Fig.3

SC79 increases NO via PI3K-dependent eNOS phosphorylation in A549, primary nasal, and type II AECs. a, b Cells were treated with SC79 (10 μg/ml; DMSO only as control) for 30 min, 1 h, and, 2 h alone or with LY294002 (10 μg/ml) for 1 h before incubating with SC79. Bars are means ± SEM; n ≥ 3 experiments. a *p < 0.05 (CTL vs. SC) 2 h and b *p < 0.05 (CTL vs. SC) and **p < 0.01 (SC vs. SC + LY) 2 h by ANOVA and Bonferroni posttest. c Cells were loaded with 5 μM DAF-FM for 60 min and stimulated with SC79 (10 μg/ml) ± LY294002 (10 μg/ml). DAF-FM intensities were normalized 14 min after SC79. Bars are mean ± SEM; n = 3 experiments. *p < 0.05 (CTL vs. SC) and **p < 0.01 (SC vs. SC + LY) by ANOVA and Bonferroni posttest. d Primary nasal cells were cultured and treated with SC79 (10 μg/ml) for 2 h before lysing for Western blotting. Bars are mean ± SEM; n = 3. ***p < 0.0001 (CTL vs. SC) by unpaired Student’s t-test. e Primary type II AECs were treated with SC79 (10 μg/ml) for 2 h before lysing for Western. Bars are mean ± SEM; n = 3. *p < 0.05 (CTL vs. SC) by unpaired Student’s t-test