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Fig. 2 | Respiratory Research

Fig. 2

From: Small-molecule Akt-activation in airway cells induces NO production and reduces IL-8 transcription through Nrf-2

Fig. 2

SC79 phosphorylates and activates Akt in A549 cells. a, b A549s were treated with SC79 (10 μg/ml; DMSO only as control) or PI3K inhibitor LY294002 (10 μg/ml) for 1 h before incubating with SC79 for 1 h and 2 h. Cell lysates were collected for Western. Bars are mean ± SEM; n ≥ 3 experiments. a ***p < 0.001 (CTL vs. SC) 2 h and b *p < 0.05 (CTL vs. SC) and ***p < 0.001 (SC vs SC + LY) 2 h by ANOVA and Bonferroni posttest. c A549s were transfected with biosensor AktAR and stimulated with SC79 (10 μg/ml). FRET (YFP/CFP emission ratio) was normalized at 14 min. Bars are mean ± SEM; n = 6. ***p < 0.001 (CTL vs. SC) by unpaired Student’s t-test. d SC79 does not induce phosphorylation of ERK1/2. A549 cells were treated with SC79 (10 μg/ml; DMSO only as control) for 2 h before harvesting for Western. Bars are mean ± SEM; n = 3 experiments

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