Fig. 1

Localization and expression of Akt isoforms and eNOS in A549 and primary nasal epithelial cells. a–c IF was performed for Akt and eNOS as described. a Secondary antibody only control, b Akt, localized largely to the cytosol and c membrane-localized eNOS, a palmitoylated membrane-associated protein. Scale bar is 10 μm. d–f A549 cells and primary nasal cells (submerged and ALI) were lysed for qPCR to measure levels of Akt isoforms. Bars are expression relative to GAPDH (2^−ΔCT; mean ± SEM); n = 3 experiments