Fig. 4
From: Dual inhibition of αvβ6 and αvβ1 reduces fibrogenesis in lung tissue explants from patients with IPF
Pulmonary collagen deposition and Smad3 phosphorylation in bleomycin-challenged mice receiving dual αvβ6/αvβ1 inhibitor. A SHG microscopy images of interstitial collagen deposition (blue) in lung tissue sections collected from sham-challenged or bleomycin-challenged mice (vehicle or 500 mg/kg PLN-74809). B Morphometric analysis of interstitial fibrillar collagen deposition from SHG imaging, and C pSmad3/Smad3 ratio in lung tissue from bleomycin-challenged mice treated with PLN-74809 (100, 250, and 500 mg/kg) or vehicle was compared to sham-challenged mice. PLN-74809 was dosed orally (100–500 mg/kg BID) in mice from 7 to 21 days post-bleomycin-induced lung injury. A Representative images show both fibrotic interstitial fine collagen fibers (blue) and denser normal structural collagens surrounding airways (red). B, C Data presented as box and whisker plot with minimum, 25th, 50th, 75th percentile, and maximum values indicated. *P < 0.05; **P < 0.01; ****P < 0.0001. BID: twice daily; Bleo: bleomycin; PBS: phosphate-buffered saline; pSmad3: phosphorylated Smad3; SHG: second harmonic generation