Fig. 3
From: Dual inhibition of αvβ6 and αvβ1 reduces fibrogenesis in lung tissue explants from patients with IPF
Mean (± SD) αvβ1 protein levels measured in A lung tissue from healthy subjects vs patients with IPF and B healthy vs fibrotic mouse lung tissue (21 days post-bleomycin challenge). Efficacy of integrin inhibitors of αvβ6 (3G9), αvβ1 (Compound A), or αvβ6/αvβ1 (PLN-74809) at blocking (C) normal human bronchial epithelial cell, D normal human lung fibroblast, and E IPF lung fibroblast adhesion to TGF-β LAP as determined by cell impedance assay. One representative donor cell plotted in (C–E) (mean [± SD] for n = 3 replicate measurements). Pan-αv integrin- and pan-β1 integrin-inhibiting antibodies were also used to demonstrate αvβ1-mediated adhesion of normal and IPF lung fibroblasts to LAP (D, E). Ab: antibody; conc: concentration; Cpd A: Compound A; IPF: idiopathic pulmonary fibrosis; LAP: latency-associated peptide; SD: standard deviation; SMi: small-molecule inhibitor; TGF-β: transforming growth factor-β