Fig. 6

Effect of combination ABCC4 and PDE4 inhibition on LABA/GCS responses in human airway epithelial cells exposed to TLR ligands. An inflammatory stimulus of Poly I:C (1 µg/mL) was used to model a viral challenge in human airway epithelial cells. Combinations of LABA/GCS (budesonide [10 nM]/formoterol [0.01 nM]), ABCC4 inhibitor (Ceefourin-1[10uM]) and PDE4 inhibitor (roflumilast [1uM]) were performed with Poly I:C exposure and the cytokines, A IL-6, B IL-8 were measured (n = 6). Then, using the same conditions but changing the inflammatory stimulus to imiquimod (100 µg/mL) C IL-6, D IL-8 were measured (n = 4). All data are represented as percentages normalized to Poly I:C/imiquimod ± SEM. (p < 0.01^##, p < 0.001^### compared with positive control, and p < 0.05*, p < 0.01**, p < 0.0001**** compared with Poly I:C/imiquimod + budesonide/formoterol)