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Fig. 1 | Respiratory Research

Fig. 1

From: Potentiation of long-acting β2-agonist and glucocorticoid responses in human airway epithelial cells by modulation of intracellular cAMP

Fig. 1

Characterization of ABCC4 expression and localization patterns at the gene and protein level. In situ hybridization of ABCC4 RNAscope™ probe in human lung under low (10X) (A) and high (40X) (B) magnification. Red puncta are representative of ABCC4 gene transcripts with nuclei counterstained blue. Representative image of n = 10. C Gene expression data for 616 healthy subjects with no history of smoking or chronic respiratory disease. D Healthy samples with metadata defining sex were further divided into male (N = 227) and female (N = 103) groups and plotted separately as blue and orange-outlined box plots, respectively. For both C and D, log2-transformed expression values were plotted as box plots. The dashed line at zero represents the global baseline of expression for the entire set of genes. Immunohistochemistry of ABCC4 in human lung under low (10X) (E) and high (40X) (F) magnification. Representative image of n = 10. Pink/red staining is representative of ABCC4 protein with nuclei counterstained blue. G ABCC4 protein expression human airway epithelial cells via immunoblot. Lane 1: Protein Ladder, lane 2–4: HBEC-6KT cell lysate (n = 3 independent cultures), lane 5–7: Calu-3 cell lysate (n = 3 independent cultures), lane 8–9: Primary cell lysate (n = 2 independent donors). H A total protein stain was performed to demonstrate protein loading levels for the ABCC4 immunoblot

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