Fig. 1

The expression of EBI3 was upregulated in bleomycin-induced pulmonary fibrosis model. A Volcano graph showing the differentially expressing genes in the lung between nontreated and bleomycin-induced pulmonary fibrosis model mice. B, C qPCR analysis of EBI3 (B), Il12a and Il27a (C) in the lung tissues from nontreated and bleomycin-induced pulmonary fibrosis model mice. Actin was used as loading control and for the relative normalization (n = 5). D The serum was collected at the indicated time points. The protein level of EBI3 and IL-12p35 were measured by ELISA (n = 5). E qPCR analysis of EBI3 and Il27a in the peripheral blood mononuclear cells from the healthy donors (n = 18) or patients with pulmonary fibrosis (n = 17). Data are representative of three independent experiments. Data are presented as mean ± SEM. Significance was determined by two-tailed Student’s t test. **P < 0.01